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LABORATORY OF BIOACTIVE SUBSTANCES

GosNIIsyntezbelok

Address: 109004 Moscow,
B-Communisticheskaya st., 27.
Telephones: (095) 912-7083, 912-7009;
T./fax: (095) 912-6741
E-mail: penker@mail.ru; biotri@orc.ru


Labs Chief

Doctor of Sciences, Prof. Avchieva Penker Babaevna.

Themes to choose:

SPHERE OF ACTIVITY
SCIENTIFIC DEVELOPMENTS
  1. CITRIC ACID
  2. CAROTENOIDES - Beta-CAROTENE, LYCOPENE. LIPIDS
  3. ALL-ROUND BIOMASS Bl. trispora PROCESSING
BIOLOGICALLY ACTIVE FOOD ADDITIVES
ECOLOGY. ENVIRONMENTAL PROTECTION
BIOSUBSTANCES FOR PLANT PROTECTION
REMEDIES AGAINST LARVA MOSQUITO
SOIL PROTECTION FROM WIND EROSION AND INCREASING OF SOIL FERTILITY
METHODS OF SOIL DECONTAMINATION FROM MINERAL OILS, BENZOL AND PHENOLIC COMPOUNDS POLLUTION
NEW LINES OF SCIENTIFIC DEVELOPMENT

Sphere of activity

Laboratory of biosubstances is one of the research subdivisions of the State R&D Institute of Protein Substance Biosynthesis (GosNIIsyntezbelok). The institute numbers experimental facilities with necessary equipment to process microorganism fermentation and separation of the products of microbial synthesis along with the extraction of biomass in organic solvent process, and a set of production ranged microorganisms.

8 plants have been using the labs know-how to produce the feed biomass and different bioactive substances.

The Institute has also the set of production-ranged microorganisms aimed to deal with the problem of different organic and mineral compounds i.e. methanol, petroleum pollutions, heavy metals, agricultural and industrial wastes.

The laboratory is specialized in the field of microbiological biotechnology. Its main research and application activity is connected with the development and production of microbial synthesis products for food industry, medicine, cosmetology, cattle-breeding, agriculture, environmental protection.

Labs structure includes:

Researchers team comprising specialists in the field of biotechnology, microbiology along with production-engineers;
biotechnological facilities for biomass processing;
commercial company dealing with sales of the labs developments.
E-mail: smartru@online.ru
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LABS SCIENTIFIC DEVELOPMENTS

  1. Citric Acid

The laboratory boasts to have bred high-productive cultures of the Aspergillus niger fungus, which produces citric acid and have developed the process of citric acid production both at a batch and non-stop fermentation of the producing agents.

Raw material for citric acid production serve beet or cane molasses, raw sugar and starch.
Technology envisages production of crystalline citric acid, 50%- water solution of citric acid, and citric acid salts - sodium citrate and potassium citrate.
Both the Aspergillus niger fungus cultures and process modes have passed testings and were successfully introduced for the citric acid production at Belgorodsky plant, Russia.
The processo of citric acid production comprises following main stages:
preparation of nutrient medium;
production of seeding material;
fermentation;
citric acid precipitation;
calcium citrate decomposition;
vacuum-evaporation of citric acid solution;
crystallization of citric acid solutions;
drying of crystals and packaging.
The process of citric acid production envisages utilization of the citric acid production wastes.

The Lab together with Belgorodsky Citric Acid Plant is in a position to start cooperation with all parties concerned in development and setting up of the citric acid production.

The cooperation envisages:

transfer of the cultures producing agents along with the production process of the citric acid and its salts;
adjustment of the process to customers raw materials;
making specification and choice of equipment for the required production capacity production;
preparation of design documenmtation for setting-up a plant;
choice of a developer with necessary experience in designing plants for citric acid production;
training of specialists at the plant for citric acid production;
assistance while putting a plant into operation.

Further microbiological, biochemical and physico-chemical proprieties of the citric acid synthesis can be found in the book published jointly with Citric Acid Plant under the title:

"DIRECT SYNTHESIS OF THE CITRIC ACID AT A BATCH AND NON-STOP FERMENTATION PROCESS OF A.niger FUNGUS."

Authors: Avchieva P.B., Kozlov V.P.

To order the book please apply to:
109004 Moscow, B- Communisticheskaya st., 27, GosNIIsyntezbelok.
Telephones: 912-7083, 912-7009 t./fax 912-6741
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  1. CAROTENOIDES - Beta-CAROTENE, LYCOPENE. LIPIDS

The laboratory makes researches at choicing of the producing agents and development of the carotenoides over-active synthesis process, beta-carotene and lycopene, in particular.

The conditions for joint cultivating of (+) and (-) Blakeslea trispora fungus cultures were defined, possessing over-active synthesis of beta-carotene and lycopene with maiz-soybean and mineral nutrient media. Biomass produced contains 15 - 50 mg/g ADS of carotenoides in total, with about 50% lycopene.

MAIN ADVANTAGES OF NEW CULTURES AND OF THE PROCESS:

Depending on cultures choice and cultivating mode, the biomass can accumulate from 40 to 70% of lipids, and contain beta-carotene, lycopene along with such valuable antioxidants as vitamins E and K, ubiquinones - Q9 and Q10, also the ergosterene.

Fractional composition of lipids of Bl.trispora fungus
Name COMPOSITION, % of total lipids
Phospholipids
Monoglicerides
Diglicerides
Free fatty acids
Triglicerides
Ergosterene (provitamin D2)
Beta-carotene
Lycopene
Vitamin E
Vitamin K
Total ubiquinones (Q9, Q10)
7,0 - 10,0
1,5 - 4,0
6,0 - 7.9
18,0 - 19,5
30,0 - 55,5
2,5 - 7,0
10,0 - 35,0 mg/g lipids
10,0 - 35,0 mg/g lipids
160 - 190 mg/100g lipids
0,2 - 0,4 mg/g lipids
3,0 - 16,0 mg/g lipids

With regard to content of fatty acids, the lipids of Blakeslea trispora fungus are close to vegetable oils, to sunflower oil, in particular. The content of essential fatty acids makes from 53 to 85%, that significantly increases the value of biolipid complex.

Fatty acid content of lipids in Blakeslea trispora fungus biomass, % of fatty acids total content.
Composition Content
12:0
C 14:0
C 16:0
C 17:0
C18:0
C 18:1
C18:2
C18:3
Unidentified
Total non-saturated fatty acids
No
1,86-2,98
10,36 - 12,65
1.0- 2,73
3,14 - 4,05
14,27 - 16,69
37,37 - 60,87
5,47 - 7,94
To 100
57,00 - 85,00

The biomass also contains almost complete set of B-group vitamins and complete set of essential aminoacids.

Content of vitamins of B group in biomass of Bl. trispora fungus
Composition Content, mg/kg.
Thyamine (1)
Riboflavine (2)
Pantothenic acid (3)
Nicotinic (5)
Pyridoxine (6)
Vitamin 12
4,12-4,18
215-220
70-80
27-32
24-28
42-44

Positive experience of many years has been gained in the application of fungus biomass as a vitamin feed additive in a ration of live farmings - poultry, pigs, sheep, cattle, furry animals.

And in all cases the use of biomass was accompanied by significant increase of the productive ability along with the quality improvement.

Bringing biomass enriched both with carotenoides and the whole range of natural vitamins, aminoacids, antioxidants and other valuable components into the ration of live farmings makes excessfull further balancing of the feeds with artificial vitamins, aminoacids, fatty acids and mineral compounds.


BIOMASS EFFICIENCY IN THE RATION OF PETS AND FURRY ANIMALS.
High efficiency of the feed additive has been proved while using fungus carotene-bearing biomass for feeding minks. Corresponding researches were carried out with the group of pedigreed and young minks in slaughter age.
Main criteria taken for the estimation of additives influence were:
reproductory ability;
cubs yield;
minks size;
fur quality, in particular, fells size, quality test, loss of fells at defects.

Researches has proved that carotene-bearing biomass brought into mink ration as much as 0,5 g/ a head a day, has resulted in 100% of minks giving successfully cubes compared to 73% in groups under control. The yield of cubes has also shown an average increase of 20%.

The highest quality performance was achieved in the groups, which received carotene-bearing biomass. These groups proved to have the biggest-sized fells - 120,8% compared to 114,3% in groups under control along with the lowest losses of fells at defects - 6,8%, compared to 8,6% in groups under control.

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  1. ALL-ROUND PROCESSING OF BIOMASS Bl. trispora

The laboratory developed all-round processing of Bl.rtispora fungus biomass, including extraction and treatment of lipids.

Main proceesing products are:

lipids (product trade name - "LIPIDOVIT")
phospholipids;
carotenoides (beta-carotene, lycopene);
fatty acids;
ergosterene;
ubiquinones (Q9 and Q10).

All the substances mentioned above have wide application in food industry, medicine, cosmetology etc. The biomass remaining after the extraction process is used as feed additive for various species of animals, furry animals, birds and fish.

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BIOACTIVE FOOD ADDITIVES

Two kinds of bioactive food additives based on Ubiquinone 10 (Q10) and other natural antioxidants were developed and certified in Russia:

COENZYME - 10 (Q10);
QUITEN-VIT (Q10-vit).

COENZYME - 10 is a 2%-solution of Q10 in olive oil. Researches made on assimilibility of ubiquinone - 10 both for peroral taking in crystalline form and dissolved in various kinds of oils have shown the best results, when used with cotton and olive oils, which is explained by high content of vitamin E and monounsaturated fatty acids.

Q10 - is a unique antioxidant of each human cell serving the energy supply process. Its content influences normal functioning of a cell and the organism itself. The necessary amount of Q10 necessary for normal functioning is generated in organism up to the age of 35-40. Then Q10 synthesis rate gradually slows down and the ageing of the organism begins.

Our heasrt is the first, which is influenced by Q10 shortage. Reduced energetic ability of cells can no longer ensure proper functioning of our heart. Taking Q10 with food provides protection of heart ischemia, reduces the risk of heart attack, significantly suspends cells ageing process and increases physical condition of an organism on the whole.

Owing to Q10 antioxidant and energy supplying proprieties, COENZYME-10 is successfully used for medical treatment and prevention of the following diseases:

COENZYME-10 is also recommended for increase of energetics of an organism, prophylaxis of cardio-vascular system diseases, strengthening of immune system, normalization of metabolism, general sanation of an organism and increasing lifetime.

"Q10- vit" is a complex of natural antioxidants- ubiquinone Q10, lycopene, beta-carotene, vitamins E and K, along with the unsaturated fatty acids. The main application of "Q10-vit" is medical treatment and prophylaxis of oncologic and liver diseases.

The basis of the medicament make Q10 and lycopene. Expediency of Q10 application, antioxidants and biologically active substances is conditioned by the fact, that the prophylaxis and medical treatment of serious diseases is feasible with provision of the organism with necessary energy only.

LYCOPENE is a natural antioxidant, synthesized from plants, alga and fungi. Recent researches have found out availability of lycopene in human's plasma, being known in bigger concentrations as compared to other carotenoides.
Additionally, recent researches have proved the antioxidative properties of lycopene to be much higher as compared to the regular antioxidant vitamin E.
The essence of "Q10-vit" application lies in the ability of lycopene to bind and neutralize atomic oxygen and free radicals, which come into reaction with the cells genetic material and cause malignant neoplasms, intensification of ageing process and bringing down of ummune system.
Efficiency of lycopene application for the prophylaxis and medical treatment of oncologic diseases increases significantly if used in combination with other antioxidants and vitamins of "Q10-vit", in particular, with beta-carotene, vitamins E and K.
Vitamin K as component of the preparation serves different importanrt purposes: it provides for re-compensation of its own deposits in the organism, often almost exhausterd in the course of the therapy of oncologic and cardio-vascular system diseases by different vitamine K destroying medicaments.
Additionally, vitamin K has proved recently to be able to fight choosen oncologic cells. "Q10-vit" also contains such important essential fatty acids as linoleic and linolenic acid, the absence or deficit of which allows cholesterol and saturated fatty acids to make complex ethers, that in its turn tends to accumulate itself in blood and make deposites on artery walls, forming thus cholesterol plates being the reason of atherosclerosis process.
Linoleic and linolenic fatty acids and cholesterol form complex ethers, which oxydate during metabolism and produce low-molecular substances with light removal rate.

Based on the proprieties of "Q10-vit" components, the preparation is applicable for the prophylaxis and medical treatment of the following diseases:

Consultations on prophylaxis and medical treatment of various diseases with the help of the medicaments "COENZYME-10" and "QUITEN-VIT" including sales thorough the medical centers and drugstores is available in "The Center of Modern Medicine" at the Academy of Defence Industry, Russia; phones: (095) 140-9147; 444-9866.

WHOLESALES DELIVERIES AT THE MANUFACTURERS PRICE:
tel/fax (095) 912-6741; E-mail: penker@mail.ru; biotri@orc.ru

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ECOLOGY AND ENVIRONMENTAL PROTECTION

One of the most prospective current trends of bootechnology is production of biopreparations to fight pathogenic microorganisms and vermins, along with sanguirous insects - carriers of differdent infectious diseases.

Biopreparations made on the basis of natural cultures of microorganisms make a good alternative to chemical preparations polluting our environment.


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BIOPREPARATIONS FOR PLANT PROTECTION

The preparations developed by the laboratory serve protection of crop production from diseases as well as for the stimulation of plant growth and for the increasing the crop capacity. The preparations are developed on the basis of bacteria Pseudomonas putida, isolated from soil of rhizospheric tomato zone and of Bacillus subtilis, isolated from soil of green-houses in Moscow region.

The essence of biological methods to fight crops diseases lies in natural phenomena of parasitism or antagonism among microorganisms inhabiting crops and soil.
The effects made by the preparation include the competition for nutrition, efficient colonization of rhizosphere and leaf surfaces, antibiotic substance synthesis, siderophores, and that of substances stimulating crops growth.


Therefore, biopreparations to fight crops diseases allow to solve the problems of keeping the crop, increasing its quality and protection of environment at the same time.


Field of biopreparations application.

Preparations on the basis of bacteria Pseudomonas putida and Bacillus subtilis posess high antibacterial and antifungal activity in relation to phytopathogenic microorganisms, which are causing pathogebnese of tomatoes, cucumbers, carrots, potatoes, beet, rice, wheat and other agricultural crops. The preparations are especially efficient for the treatment of seeds in green-houses with excessive humidity and high temperature rates, thus creating favourable conditions for the development of pathogenes.
Treatment of crops seeds stimulates germinating ability and further growth of young shoots. Producton of crops when treated marks up an average increase of 25%.

Production of preparations.

Process of biopreparation production envisages growing of bacterial cultures on mineral medium or yeast-polysaccharide medium in aseptic conditions at 28 300C during 30 - 48 hours. As a result the suspension is produced of yellow-green color with specific bacterial odor. Cell titre of the producer in final preparation is 108 cell/ml.

Application of the preparations.

Biopreparations are applied according to the table. The preparations are dissolved in required amount of water and thoroughly mixed. Treatment of seeds, plants and soil is to be made with fresh-prepared solution.

Object of treatment Preparation of working solution Conditions of treatment
Treatment of seeds 1 ml of the preparation per 1 L of water

In the produced solution the seeds are held for 30 min.
Treatment of seedlings:

At the stage of 2 seedlobes and 4 true leaves

At the stage of seedlings with the height to 1 m



1 ml of the preparation per 1 L of water

1 ml of the preparation per 10 L of water


Treatment is made under the root by watering.

Treatment of seedlings is made on leaves and under the root.

Expenditure of working solution is analogous to water consumption as at watering of plants.

Treatment of soil 10 ml of the preparation in 1 L of water

In 1-2 weeks before planting out the seedlings in terms of 500-1000 L per 1hec. of soil.
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THE PREPARATION TO FIGHT THE LARVA MOSQUITO

The biological preparation ANTINAT is developed to fight the larva of different mosquito species.The preparation is produced on the basis of natural culture Bacillus thuringiensis var.israelensis -14, possessing high mosquitocidic activity against larva of urban, basement and malaria mosquitos (Aedes, Culex, Anopheles).

Active substance of the preparation is crystalline endotoxin, synthesized by bacteria cells.
ANTINAT is a liquid paste of beige-grey color.
It is intended to fight mosquito larva in open waters (ponds, puddles, reservoirs with water, etc.) and also in basements of dwelling houses.
It is used as water suspension for even spraying the object's surface.
ANTINAT concentration and consumption of working solution is dependant from the type of the treated object and the age of larva and can comprise 0,5-4 mg/l.
It effects as intestine insecticide, causing the paralysis of the intestine blocking of nutrition process of the larva in several minutes after the toxin is swallowed up.
The efficiency of the preparation results in destruction of 95-100% of larva within first 24 hours.
The preparation is strictly developed to fight exclusively mosquito larva, making no harm to humans, animals, birds, fish and useful hydrofauna.

Compared to similary preparations ANTINAT is notable for its 100% ecological safety, effectiveness, high biological activity, being saving and friendly in application.
Production process of the preparation is simple and easy. The preparation can be produced on any microbiological equipment. The fermentation takes 28-40 hours.


For consultations and instructions on application and purchase of "ANTINAT" please apply to: phone- 912-6741; 748-4045.

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SOIL PROTECTION FROM WIND EROSION AND INCREASE OF SOIL FERTILITY

As a mater of a fact, the problem of soil protectiom from wind-and water erosion is typical for all countries with hot dry climate. Millions of hectares of fertile soils are blowen-and-washed out each year, destroying thus future crop yields. The demand for technologies, capable to protect soil, to increase its fertility and to preserve the environment is great. Especially, when taken into account, that actual state of science has no complex approach to the solution of this problem.

In many countries this problem is solved by the application of the expensive acryl-bearing chemical substancess with application techniques requiring special equipment. These preparations are also aggressive and harmlfull to the chemical composition of soil, showing the period of decomposition of some inorganic compounds, which are formed as a result of treatment of soil with "glue" compositions, of dozen of years.
In addition, along with the problems of water-and-wind erosion, modern agriculture features a number of degradation processes, occuring in soil: high level of soil dehumification, acidification, wide spread re-thickening of soils, salinization, and also the expansion of areals with high concentration of various chemical weed-killers accumulated due to the excessful application of the latter. All this causes significant reduction of arable soild area, decreases crops productivity, deteriorates quality and nutrition value of agricultural products.

The laboratory of bioactive substances has elaborated the process, which at one time protecs soil from wind erosion and increases its fertility, that is based on the application of the "composition" of biologically active compounds, involved in soil humus-formation with a crust forming layer on the surface of sand and soil.

Main components of the "composition" are biologically active substances, organic, nitrogenous and mineral compounds, and also complex-forming substances with the prolonged activity effect. One of the components advises porous structure and actively absorbs water and moisture vapours from the air. Maximum value of water vapour absorbtion is 110-120 mg/g.

According to the developed process, aiming to increase application efficiency of the "compositions" components and also to achieve the crust-forming layer on the soil surface, one of the components is exposed to alkaline hydrolysis under soft conditions. As result a viscous homogeneous mass with adhesive proprieties is achieved. While mixing this mass with the second component in 1:1 ratio, neutralization of the mass takes place with further pH value close to a neutral rate.

Amount of "composition" for introduction into the soil depends on the quality of soil itself, on the level of its cultivation and erosion-resistant factors . In order to prepare a well-balanced ratio of biogenic elements in soil, the "compositions" compounds should be introduced into the soil in an amount ensuring the ratio org.:Nminer.:P:K of main biogenic elements to be as much as 40:1:0,5:1.

The process envisages two variants of soil treatment:

  1. Increase of soil fertility.
    Estimated amount of the "compositions" components is introduced into the soil with further tillage into the soil layer.

  2. Protection of soil from wind erosion and increase of soil fertility.
    Along with the introduction of of the components into the layer of soil, the surface will be covered with a thin layer of the "composition".

The "composition" of biologically active substances can be applied two-three times to speed up the process of re-using erosive soils for agriculture and to increase fertility of the already exhausted soils.

Owing to its structure, the crust-like surface, resulted from application of the "composition" in the soil, prevents fertile layers of soil from wind erosion, at the same time letting moisture and air penetrate into the inner fertile layers.
The crust-like surafce itself advises swelling and moisture retention proprieties and prevents while wetting losses of fertile layer due to water erosion. Owing to porous and organic structure, the surface does not hinder germination and growth of shoots and stimulates both processes as well.

Control data for processes in soil before and after the treatment:

total carbon content;
total nitrogen content;
content of nitrogen- ammonium, nitrate, nitrite;
content of phosphorus, total and movable;
content of potassium, total and movable;
content of moisture in soil layer;
content of total bacterial seeding;
content of ammonifying bacteria;
germination of seeded (test culture) grass.

Application of the method for the increase of soil fertility and protection from wind erosion, based on the introduction of the "composition" of biologically active components in the soil contributes to:

  • that of the soil structure: the soil acqures fine-lumpous grain structure, that provides for decrease of fertile layer losses while wind-and-water erosion;
  • increase of the content of main biogenic elements(carbon, nitrogen of nitrates, movable phosphorus and potassium);
  • increase of moisture rate in soil;
  • stimulation of the germination and growth of the seeded grass;
  • activation of soil microflora development.

Researches carried out with the samples of the salinized soils have proved that biologically active compounds as "compositions" ones could be successfully used as land-improvers on greatly salinized soils.

"Compositions" components are biotechnological processing wastes, in particular, wastes of raw plant material processing and wastewaters purification, easy obtainable and reasonably priced.

The process of soil protection from wind erosion and for the increase of the soil fertility is ecologically sound, pollution-free and aiming at simultaneous solution of three ecological problems:

  • increasing of soil fertility;
  • fighting wind and-water erosion of soils and its exhaustion;
  • utilization of biotechnological processing wastes;

The process of preparation and application of the "composition" does not require using special equipment. Demand for main components is 3-15 tons/hectare, subject to place of application.

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TECHNOLOGY FOR DECONTAMINATION OF SOIL FROM OIL, BENZOL AND PHENOLIC COMPOUND POLLUTIONS

Decontamination of soils from pollutions with oil products and other organic compounds, which suppress the vital activity of biota and keep away from involving of the recultivated soils into agricultural use is the most important task of environmental protection nowadays.

To be able to make correct choice and planning of re-cultivating measures one have to master different methods of estimation of the sanitation level of the oil-contaminated objects first.
While choosing a recultivation method, the preference is given to the methods aimed at the intensification of the microbiological processes of oil hydrocarbon utilization in soil. For this sake various companies produce and use microbial preparations, containing several microorganism-cultures.

The laboratory also disposes industrial preparations along with the process of microbiological recultivation of oil-contaminated soils. However, the researches carried out recently at the laboratory have shown the possibility to activate the soils own microflore. This increases efficiency of the decontamination and makes the process more cheap and easy.

The process developed for re-cultivation of soils contaminated with oil products and phenolic compounds is based on the application of the complex of mineral salts (CMS) aiming to intensify decontamination in a way of activation of the sois own microflora.

CMS represents itself a fine-crystalline dry powder, containig easily assimilable compounds of nitrogen, phosphor, magnesium, sodium, potassium, microelements, activating enzymatic systems of hydrocarbon-oxidating and phenol-oxidating soil microorganisms.

A specially selected composition of CMS compounds attaches it with a range of specific advantages as compared to others preparations for soil decontamination:

CMS activates soils own microflora;
CMS is free from foreign microflora;
Owing to activation with soil bacteria and mineral nutrition, CMS intensifies the process of the self-growing with the wild grass on the re-cultivated plot.
CMS is resistant to rapid fluctuations of temperature;
CMS efficiency does not depend on soil salinity, pH and other contaminations.

Recommended doses and number of CMS applications depend on the level of soil contamination and on the method of CMS application into the soil. It is desirable to carry out the treatment 1-2 times, depending on the level of the object contamination at temperature not lower + 100C. CMS application for soil re-cultivation foresees the preparation of basic solution and working suspension.

Working suspension is applied on the contaminated soil by overhead irrigation with further making soil friable.
Analysis of soil samples to determine the decontamination efficiency are scheduled as follows: prior to the treatment, and in 10,20, 30 days (further if necessary) after the treatment.

In 10-15 days, with the aim to maintain maximum possible rate of decomposition of oil and other contaminations with soils own microflora it is desirable to carry out the second treatment of soil with CMS solution. Component concentration and the process of soil treatment is analogue to the first one.
Total demand for CMS at single treatment with average level of contamination is 1-5% - 10 kg/hectare, at two treatments - 20 kg /hectare.

CMS is produced in polyethelene and paper bags, 10 and 20 kg . The warranty period of indoor storage with max. humidity 80% and temperature from 40C to +50C is 2 years.
The price of 1 kg CMS is 5-10 times lower than that of 1 kg of microbiological preparation.

CMS components are non-toxic and unpathogenic, they have application in food industry and agriculture.

CMS application for soil decontamination with the level of contamination 1-5 % with oil and phenol ensures completely reduction of phenols by the 9-th day after the treatment, while content of oil products in soil during 10 days has been decreasing by 25% and 45% after 20 days since the first treatment.
The preparation was also used for the decontamination of industrial objects with the level of soil contamination with oil products of 12%.
At that within 10 days at single CMS application the content of oil products in soil was reduced by 46%.

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NEW TRENDS OF THE LABORATORY

The laboratory carries out researches on processing of water-soluble Lipids, Ubiquinone-10 and Lycopene. Samples of the mentioned substances are obtained and currently subjected to testing.

For more detailed information on practical developments of the laboratory, industrial applications, development of new processes and technologies please apply to:
Tel. (095) 912-70-83; Tel/Fax: (095) 912-6741
E-mail:
penker@mail.ru ; biotri@orc.ru

The additional information on the
biologically active additives is on a site:
http://vitamarket.com.ua

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